Abstract: The silver pomfret (Pampus argenteus) is one kind of newly exploited marine culture species in China. The nuturional characteristics of silver pomfret broodstock during the gonad development have not been reported. The amino acids of the muscle, liver and gonad tissues of the wild-caught silver pomfret (Pampus argenteus) broodstocks from Zhoushan fishery in the East China Sea were investigated with Hitachi L-8800 highspeed amino acid analyzer in this study. The changes of content and composition on amino acid in different tissues with the gonad development were also analyzed. The results indicated that the spermary in V stage of the male broodstock had the highest taurine content, 27.8 mg/g, which was significantly higher than that in muscle or in liver of the broodstocks respectively. The total amino acids of muscle, spermary and liver of the V stage of the male broodstocks were 631.4 mg/g, 544.8 mg/g and 468.0 mg/g respectively. While in female broodstocks, the taurine content (13.7 mg/g) in liver tissue was significantly higher than those in ovary and muscle tissues. The total amino acid contents of muscle and liver of the broodstocks in V stage declined significantly as the ovary developed from III stage to V stage. While the total amino acid content of ovary was stable during the ovary development from III stage to V stage. Glu, Lys, Asp, Leu and Arg were dominant in total amino acid composition of the muscle, while Glu, Lys, Asp, Val and Leu, Glu, Asp, Lys, Arg and Val, Glu, Lys, Leu, Val and Asp were dominant in total amino acid compositions of liver, spermary and ovary respectively. There were no effects of gender and ovary development stages on the total amino acid composition of the muscle. The free amino acid contents of ovaries in III, IV and V stages were 17.7 mg/g, 41.5 mg/g and 29.6 mg/g respectively. The compositions on free amino acid in ovaries were significantly variable during the development from III stage to V stage. Although the amino acids of silver pomfret broodstock at I and II stages need to be studied further, the results of this paper suggest that the total amino acid in muscle, liver and gonad and free amino acid in gonad are variable as the gonad development.