吉奥罗非鱼(新吉富罗非鱼♀×奥利亚罗非鱼)和4种近缘遗传型罗非鱼的遗传差异的RAPD、SSR比较分析

Genetic variations among a new tilapia hybrid NEW GIFT Tilapia Oreochromis niloticus♀×blue tilapia (O. aureas) and four closed genetypes of tilapia by RAPD and SSR analysis

  • 摘要: 以1种新型杂交鱼-吉奥罗非鱼(新吉富罗非鱼♀×奥利亚罗非鱼)群体,4种近缘遗传型罗非鱼奥尼(尼罗罗非鱼♀×奥利亚罗非鱼)、新吉富罗非鱼、尼罗罗非鱼、奥利亚罗非鱼为实验材料,采用RAPD和SSR技术,比较地分析杂交种间及它们同亲本间的遗传差异。RAPD方面:①群体多态座位比例(P)-吉奥(20.4%)>新吉富(18.5%),但奥利亚(21.4%)<奥尼(21.7%)<尼罗(22.3%);②Nei's平均遗传相似系数(Ip)-吉奥(0.9086)<新吉富(0.9231),但>奥利亚(0.8895)>尼罗(0.8588)>奥尼(0.8069);③香农多样性指数(Ho)-吉奥(0.1326)>新吉富(0.1103),但<奥利亚(0.1514)<尼罗(0.1742)<奥尼(0.2410);④群体两两间遗传相似系数(Ipop)-吉奥-奥尼(0.9444)<吉奥-尼罗(0.9496)<吉奥-新吉富(0.9544),但>吉奥-奥利亚(0.9383)。SSR结果:① 平均有效等位基因(Ne)数-吉奥(2.43)>新吉富(2.31)>尼罗(1.94)>奥利亚(1.18),但<奥尼(2.82);②平均遗传杂合度(H)-吉奥(0.700)>尼罗(0.570)>奥利亚(0.497)>新吉富(0.398),但<奥尼 (0.816);③ 群体的多态信息含量(PIC)-吉奥(0.661)>尼罗(0.568)>奥利亚(0.513)>新吉富(0.420),但<奥尼(0.692); ④ 依据遗传距离构建的NJ树和UPGMA树,奥利亚罗非鱼单独为一支,吉奥等4种罗非鱼为一支。总的说来,RAPD和SSR两种技术检测结果所反映的群体遗传信息一致。这表明,无论是吉奥还是奥尼,两个杂交种在遗传上都同各自的母本较近,这同作者先前揭示的形态和生长等方面的母本效应一致。

     

    Abstract: Using a new hybird NEW GIFT Nile tilapia (Oreochromis niloticus)♀× blue tilapia(O. aureaus), short term JA and closed hybrid (common Nile tilapia ♀× blue tilapia, short term AN) as well as their parents(GIFT Nile tilapia, short term XJ; common Nile tilapia, short term NL; blue tilapia, short term AL) as study materials, their genetic variations were studied by RAPD and SSR technologies. By RAPD:①Percentage of polymophism loci(P)-JA(20.4%)>XJ(18.5%),but < AL(21.4%)Ip)-JA(0.9086) AL(0.8895)>NL(0.8588)>AN(0.8069);③Shannon diveristy index(Ho)-JA(0.1326)>XJ(0.1103),but < AL(0.1514)pop)-JA-AN (0.9444)< JA-NL(0.9496) JA-AL(0.9383).By SSR:①Average effective numbers of alleles(Ne)-JA(2.43)>XJ(2.31)>NL(1.94)>AL(1.18),but < AN(2.82);②Average heterozygosity(H)JA (0.700) > NL(0.570)>AL(0.497)>XJ(0.398),but< AN (0.816);③ polymorphism imformation content(PIC)-JA(0.661)>NL(0.568)>AL(0.513)>XJ(0.420),but < AN(0.692);④The Nj and UPGMA tree based on genetic distance showed that, AL goes to one branch seperately, JA etc. 4 genotypes run togather into a branch. In general, the information by RAPD and SSR all revealed that, the hybrid, never mind JA or AN, all closed to their mother side, which is coincident with the our previous study results from morphology and growth.

     

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