壬基酚对雌鲫受体表达和雌二醇水平的影响

刘青; 魏华; 张高峰; 吴楠

壬基酚对雌鲫受体表达和雌二醇水平的影响

Effects of 4-nonylphenol on expression of estrogen receptor and level of 17β-eatradiol in vivo of female Carassius auratus

摘要

摘要: 研究了环境激素壬基酚（4-NP）在雌鲫体内的对雌激素受体的表达和雌二醇水平的影响。雌鲫腹腔注射1、50、100 mg·kg-1的4-NP或1 mg·kg-1的E2，分别在24 h和48 h提取肝脏RNA用RT-PCR查看α型雌激素受体（ER-α）和CYP1A的表达情况和提取血清用荧光免疫法测定E2浓度。结果表明：1 mg·kg-1 E2 处理组的ER表达在24 h和48 h有显著性增加（P<0.01）。1 mg·kg-1 4-NP处理组在24 h ER表达与对照组比较有极显著性增加（P<0.01），在48 h有显著性增加（P<0.05），E2浓度在24 h与对照组比较有显著性下降（P<0.05），但在48 h后有所回升；CYP1A表达在处理后24 h有显著上调（P<0.05），在48 h后有极显著上调（P<0.01）。50 mg·kg-1 4-NP处理组在24 h ER表达与对照组比较有极显著性增加（P<0.01），在48 h有显著性增加（P<0.05），E2浓度在24 h和48 h与对照组比较有极显著性下降（P<0.01）；CYP1A表达在处理后24 h有显著上调（P<0.05），在48 h后有极显著上调（P<0.01）。100 mg·kg-1 4-NP处理组ER表达在24 h和48 h与对照组比较有极显著性增加（P<0.01），E2浓度在24 h与对照组比较有显著性下降（P<0.05），在48 h有极显著性下降（P<0.01）；CYP1A表达有极显著上调（P<0.01）。随着4-NP注射量的增加，ER表达也逐渐增加，而E2浓度逐渐减少。ER表达上调效应：E2>100 mg·kg-1 4-NP >50 mg·kg-1 4-NP >1 mg·kg-1 4-NP，100 mg·kg-1的4-NP对ER表达的上调效果接近于1 mg·kg-1E2的效果。E2下降效应：100 mg·kg-14-NP >50 mg·kg-1 4-NP>1 mg·kg-1 4-NP。NP对CYP1A表达有上调作用，并且表达随注射浓度上升而增强（具剂量-依赖效应）。以上结果表明,4-NP具有弱雌激素样效应，并且能抑制鲫鱼血清中E2的水平并使ER表达上调，为4-NP在鲫鱼体内通过ER途径实现内分泌扰乱效应的可能性提供了支持。图2表1参30

Abstract: The estrogen-like effects of 4-nonylphenol（4-NP）in vivo of female Carassius auratus were investigated in this paper. We gave intraperitoneal injection in female Carassius auratus with the dose of 1 mg·kg-1, 50 mg·kg-1, 100 mg·kg-1 of 4-NP or 1 mg·kg-1 of 17β-estradiol (E2) respectively. The expression of α-type estrogen receptor (ER-α) and CYP1A in liver was evaluated by RT-PCR and the concentration of E2 in serum was measured by fluorescent immunoassay in 24 h and 48 h of injection. The results show that 1 mg·kg-1 of E2 increased expression of ER significantly both in 24 h and 48 h (P<0.01). In 4-NP treatment group, the concentration of E2 was decreased and the expression of ER was increased with the increase of 4-NP’s level. In 1 mg·kg-1 of 4NP treatment group, there is a significant increase of expression of ER at 24 h (P<0.01) and 48 h (P<0.05), the concentration of E2 was decreased at 24 h (P<0.05) yet returned slightly at 48 h, and CYP1A’s expression is up-regulated at 24 h (P<0.05) and 48 h (P<0.01). In 50 mg·kg-1 of 4-NP treatment group, there is a significant increase of expression of ER at 24 h (P<0.01) and 48 h (P<0.05), the concentration of E2 was decreased significantly both at 24 h and 48 h (P<0.01), and CYP1A’s expression is up-regulated at 24 h (P<0.05) and 48 h (P<0.01). In 100 mg·kg-1 of 4-NP treatment group, there is a significant increase of expression of ER both at 24 h and 48 h (P<0.01), the concentration of E2 was decreased significantly at 24 h (P<0.05) and 48 h (P<0.01), and CYP1A’s expression is up-regulated (P<0.01). Effect of increasing of ER’s expression is E2>100 mg·kg-1 4-NP >50 mg·kg-1 4-NP >1 mg·kg-14-NP; effect of decreasing of concentration of E2 is 100 mg·kg-1 4-NP >50 mg·kg-1 4-NP >1 mg·kg-14-NP. The effect of 100 mg·kg-1 of 4-NP on ER’s expression is close to that of 1 mg·kg-1of E2, this indicates that 4-NP has weak estrogen-like effects. And 4-NP can up-regulate the expression of CYP1A (dose dependent). That increase in ER’s expression and decrease in E2 in 4-NP treatment group provide support of 4-NP’s endocrine disruption via ER pathways in Carassius auratus. 30 Refs.In Chinese.

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