坛紫菜丝状体种质保存技术的研究
Studies on the methods of conservation of conchocelies germplasm of Porphyra haitanensis
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摘要: 采用液体培养基、固体培养基以及胶囊化冷冻法保存坛紫菜丝状体种质,探讨了不同保存条件对其生存的影响.结果表明:液体培养基保存丝状体,在低温5 ℃,培育30 d时,藻丝的增重率为-0.67%;温度为10 ℃,有利于延长更换培养液的时间,即使120 d不换培养液,丝状体也能正常生长.固体培养基保存丝状体,在5 ℃时固体平板上的细菌生长慢、数量少,但藻丝无法适应低温条件,逐渐死亡;丝状体在10 ℃固体平板上生长缓慢,但保存100 d以上仍然正常生长,达到了长期保存的目的.在胶囊化低温保存中,丝状体存活率较高,而且随着保存时间的延长,成活率没有显著的变化,保持在75%以上.Abstract: The researches on conservation of conchocelies germplasm of Porphyra hahanensis were done. Three different kinds of conservation methods (Liquid conservation, solid conservation and encapsulation cryopreservation) were compared. The result showed: 1) The weights of conchocelies germplasm increased by - 0.67% during the 30 days under the condition of temperature 5℃ and they could develop normally up to 120 days without changing culture medium under the temperature 10℃ when the method of liquid conservation was used. 2) The conchocelies germplasm did not adapte to low temperature 5℃ condition in despite of the bacterium growing slowly and rarely and the conchocelies grew normally for 100 days when the method of solid conservation was used. 3) The 75% of survival rate of the conchocelies germplasm was kept when the method of encapsulation cryopreservation was used.
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