Abstract: Use of a vitrified solut ion ( composition 10) as a cryoprotectant for the cryopreservation of loach embryos is studied in this paper. Using this vitrif ied solution, embryos were preserved at a temperature of - 196 e for 17 hours. Afterward, the preserved embryos were thawed using thawing so lution in warm water ( 38 e ) , and revival embryos were successfully obtained. During thawing, we compared 3 kinds of thawing solution compositions ( B1, B2 and B3) , and the survival rates of the preserved embryos after thawing in B1, B2 and B3 thawing solutions were 26. 7% , 15. 0% and 3. 0% respectively. After thaw ing, it took 50 hours for the development of revival embryos from the interval of blastopore closure to the interval of caudal fin appearance.