中国对虾血淋巴抗凝剂的筛选

Selection of Anticoagulant to the Hemolymph of Penaeus chinesis

摘要: 依据中国对虾血细胞电镜照片中血细胞整体形态和细胞器、细胞核的变化,研究对虾抗凝剂成分配比.初步确认了一种理想的抗凝剂配方NaCl450mM、KCl 10mM、EDTA.Na210mM、HEPES 10mM(pH 7.45).它能够良好的保持细胞的整体形态以及细胞器、细胞核的形态.

Abstract: The morphology of the whole hemocyte and the variation of organelle and nucleus were observed by TEM in order to determine the composition of shrimp anticoagulant. A confirmed desirable anticoagulant is composedofNaCl 450mM,KCl 10mM,EDTA.Na210mM,HEPES 10mM (pH 7.45),which can maintain successfully the form of the whole hemocyte ,organelle and nucleus.