海水中几种金属离子对中国对虾幼体_省略_内碱性磷酸酶和ATPase的影响
Influences of metal ions in seawater on activities of alkaline phosphatase(AKP) and ATPase in mysis and postlarvae of Penaeus chinensis
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摘要: 向经过螯合剂Cephlex 100处理的海水中添加金属离子,进行培养中国对虾糠虾幼体和仔虾幼体,观察金属离子对变态发育及其体内碱性磷酸酶和ATPase活性的影响.结果显示当海水中Cu2+、Co2 、Mn2 浓度分别为40、40、20μg·L-时明显激活糠虾幼体体内碱性磷酸酶的活性,但大于此浓度时则使该酶活性降低;Cu2 低浓度时能够提高仔虾体内碱性磷酸酶和ATPase的活性,浓度在40μg·L-1时碱性磷酸酶的活性达到最高,在40～80μg·L-1浓度范围内ATPase活性明显高于对照组,较高浓度则抑制两种酶的活性.Co2 在20μg·L-1时明显提高两种酶的活性.而Ni2 只使两种酶活性降低.鉴于碱性磷酸酶和ATPase与生长发育直接相关,金属离子对对虾幼体生长的影响与对两种酶活性的影响基本致,因此体内这两种酶活性的高低可作为判别环境因素是否适合对虾幼体生存的指标.Abstract: The influences of some metal ions in seawater treated by Cephlex 100 on metamorphosis , growth and activities of alkaline phosphatase (AKP) and ATPase in mysis and postlarvae of Chinese prawn Penaeus chinensis werestudied. The results showed:Alkaline phosphatase (AKP) and ATPase are two kinds of enzymes that are related to growth and development of animals. Cu2+ , Co2 and Mn2 whose concentrations were 40 , 40 , 20μg ·L -1 respectively could increase the AKP activity significantly in mysis , but higher concentrations decreased the AKP activity. Low Cu2 concentrations (40 -80μg ·L -1) increased the activities of AKP and ATPase significantly ( P < 0. 05) in postlarvae , and Co2 at 20μg·L -1 increased the activities , but Ni2 could not activate these two kinds of enzymes. Because changes of AKP and ATPase activities were relative to the growth directly, the activity of AKP and ATPase could be the indicators for assessment of the physiological condition of the larvae.
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