硒酵母的培养及其养虾效果
Culture of selenium yeast and its application of the yeast to shrimp culture
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摘要: 对五株假丝酵母菌株进行利用淀粉废水和转化硒元素的筛选试验, 其中CY- 173 菌株所得的蛋白质 含量和蛋白态硒总量最多。对其进行耐高温试验, 在33~ 45 e 范围内, 菌株都能生长, 但超过40e 生长速 度开始减缓, 45e 时明显受到抑制。在6L 标准发酵罐内进行pH 与温度条件试验, pH 在3~ 5 之间, 菌株生 长差异不显著; 在33~ 40 e 范围内, 菌株都能较好生长, 但以37e 的蛋白质含量与蛋白态硒总量为最高。 将所得的硒酵母替代部分鱼粉用于养殖中国对虾, 结果三组硒酵母饲料( 含10%、20%、30% 硒酵母) 的养 殖效果均优于对照饲料, 其中10%硒酵母添加量的饲料( 含硒0. 6@ 10- 6) , 其饵料系数最低, 为2. 56, 比对 照组的2. 95 减少了13. 3%。Abstract: The selecting experiment on ut ilizing starch wastewater by f ive Candida tropicalis strains and their transforming of selenium were studied. The result s showed that the strain of CY-173 produced more protein and protein-form selenium than the others . The temperature tolerance test proved that the bacteria were able to grow upto 33- 45 e . The growth rate slowed down when temperature were above 40 e and the growth was inhibited at 45 e . An experiment concerning the ef fects of pH and temperatures on their growth rate and the amount of protein produced was also carried out in a standard 6-liter tun. The dif ference of growth rates of the strains was not significant at pH 3- 5 , and they could grow normally at 33- 40 e . The amounts of protein and protein-form selenium produced by the strains reached the highest level at 37 e .Feed with the seleniumrich yeast was used in the culture of Penaeus chinensis . The results showed that the three groups fed with the selenium-rich yeast ( containing 10% , 20%, 30% selenium yeast ) had better effect s than the control group , and the group with 10% of the yeast ( containing 0. 6 @ 10 - 6 selenium ) had the lowest feed rat io, 2. 56, reducing 13. 3% compared with the control group.