Abstract: The L9( 34) table of orthogonal design method was used to formulate nine diet combinations, which were employed to determine the requirements of protein, fat, mineral and general energy for eel, Monopterus albus. The results indicated that protein and general energy were the major elements that affected the growth of Monopterus albus . The optimum percentage of protein, fat, mineral and carbohydrate were 35. 7%, 3% - 4%, 3% and 24% - 33% respectively. In the diet of Monopterus albus, the relative general energy and energy..protein ratio( E/ P= kJ..g - 1 ) were 11. 50- 12. 50 kJ..g - 1 and 31. 6- 38. 9 respectively.