Abstract: The virus nucleic acid was isolated from disease Siniperca chuatsi , and extracted with RNase, DNase and Mung bean nuclease respectively. The tests indicated that the virus genome is dsDNA. Part of the genome of Siniperca chuatsi virus were successful amplif ied by random primer ( contained the enzyme position of EcoR .. ) . The PCR products were recovered from low melting-temperature agarose and cloned in pUC19 plasmid. Three kinds of recombinant plasmids have been identified with EcoR ... Two kinds of it have been sequenced. There were 369bp and 450bp in size. It was demonstrated that there were not homology sequences against tho se in GenBank.