Abstract: The bienzyme metho d used to hydr oly ze f our aquat ic species such as Sesacma, Per na vi ridis L. , Pinctada mar tensi i D. , and H arengula z unasi B. f or the prepar at ion of aquat ic Hydroly ze d Animal Pro te in( HAP) w as studied. The bienzyme invo lves B. Subct ls neutr al pro teinase w ith act ivity 105 L/ g and pepsin w ith act ivity 3 000 L/ g. The deg ree of hydr oly sis by the bienzyme metho d wa s 43. 4% fo r Sesacma, 82. 0% f or Perna vi ridis L. , 82. 6% fo r Pinctada mart ensii D. , and 93. 6% fo r H arengula z unasi B. . More over , the deo do rizat io n and debitterizat ion of hy dro lyzed so lut io ns by means of yeast pow der and f lavo ure ase w ere also studied in this papaer. Thro ugh the pro cesses o f deodo rizat ion , debi tterizat ion, and concentr atio n o r spr ay dr ying, a hig h qua lity pr oduct o f HAP with go od smell and de licia te f lav our w as prepared, w hich can be used as fo od addit ive.