热休克法诱导日本沼虾四倍体的初步研究
A PRELIMINARY STUDY ON INDUCTION OF TETRAPLOIDY IN THE FRESHWATER PRAWN MACROBRACHIUM NIPPONENSE BY HEAT SHOCK
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摘要: 以热休克抑制受精卵第一次卵裂, 进行日本沼虾四倍体诱导实验。热休克温度在38、39、40 和41 ℃, 于产卵210 ~ 230 分钟后, 处理1 ~ 2 分钟均可获得四倍体胚胎, 40 ℃处理1 .5 分钟, 四倍体胚胎诱导 率达36 .8 %, 38 和39 ℃处理2 分钟所获得的嵌合体胚胎比率大于四倍胚胎比率, 而热休克温度40 ℃时, 四倍 体率显著高于嵌合体比率。41 ℃处理时由于有丝分裂异常导致胚胎死亡率高, 更适宜的处理时间有待进一步 研究, 以便提高四倍体育成率。Abstract: Heat shocks were used in this experiment for inducing tetraploids through inhibition o f the first cleavage division in the freshwater prawn Macrobrachium nipponense .Te traploidy prawn embryos had been produced by treating egg s at either 38、39 、40 o r 41 ℃ for 1 ~ 2 minute duration from 210 ~ 230 minutes shortly af ter spawning .At 40 ℃ , shock lasting 1.5 minutes yield 36 .8% tetraploidy embryos .The percentage of mosaics were significantly higher than that of tetraploids at shock of either 39 ℃ for 2 minutes.With shock 41 ℃, the mo rtality of embry os was high due to abono rmal kary okinesis at mitosis.Optional treatment time is needed fo r further investigating in o rder to yield hig h percentages of te traploids .
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