Abstract: The regeneration characteristics of the tissue fragments from Ca-loglossa leprieurii thallus were studied and the method of rope-adhering seedlings regenerated from tissue fragments was developed. Tha results are as follows:1 .The tissue fragments from v egetative leaches poasess great. capacity of rege-neration.2. The suitable site of the fragment is 50 to 100 cells.3. The specific gravity of the culture medium is 1 .010-1.025, the optimum va-lue is 1.015. 4.The proper amount of chitin added in the culture medium can enhance the regeneration and raise the adhension ratio of the pellets. Under ordinary tempera-ture and light for 15-20 days of culture, the regeneration buds became visible, after another 30 days of culture in a larger water body with aeration, the algae could grow to normal adult size. The method is easy to operate, and it will probably be-come a useful technique for seaweed production.