Abstract: Lactate deiiydrogenase (LDH) isazymes of 20 species of Cyprinidae fish were analysed with disc palyacrylamide gel electropharassis. In Cultrinae, there are sei eras different LDH isozyme仙enatypes which reflect the intergeneric relationships in the subfamily levol.According to their i9ozymes phenotypes, we divide them into four groups, vrhich show evolutionary divergence and welt agree with the results of systematic studies based on their morphological and anataznical characteristics. While in fishes of Cultrinae, Xenocyprininas and Hypophthalmichthyinae, tha differences of LDH isoayme phenotypes may be classified into two primal branches. One branch contains mast species of Cultrinae, they all Shaw same electropharetic ruohility of LDH-A isaayme. This character showns that the species of this branch are closely related to thoso of Leuciscinac. The second branch consists of the species of Xenocyprininas and Hypophthalmichthyinae which show same eleetrapboretic mobility of LDII-B, isozyme, this result indicates the close relationship between species of this branch and other species of Cultrinae.