Abstract: To preserve the genetic resources of Chinese sturgeon(Acipenser sinensis), a kind of fish on the edge of extinction, its blastula cells and gastrulae cells have been cryopreserved in -196℃ liquid nitrogen and gone through nuclear transplant experiment. 4 cryoprotectant solution, i.e., CP1 (12%D), CP2 (10%P), CP3(8%D+6%E) and CP4(7%+6%E) compound with 3 cryoprotectants, i.e., dimethylsulfoxide (DMSO), 1,2- propylene glycol (PG) and hydroxyethyl starch (HES), survival rates of cryopreserved cells are 47.4±4.7%, 64.4±3.6%, 54.7±4.7% and 76.7±5.7% respectively. CP4 has the best effect in preservation. It illustrates that non-penetrant cryoprotectant, hydroxyethyl starch, can improve the survival rate of cryopreserved cells. The method of cooling by chilling that cells are put in liquid nitrogen directly after balanced for 30 minutes at a temperature of -7℃ is feasible after two freezing ways are compared. The survival rates of cryopreserved embryonic cells are compared in different development stages, the survival rates of cryopreserved blastula cells and gastrulae cells are 57.1±11.2% and 64.4±11.8%. Results indicated that the survival rates of cryopreserved gastrulae cells are high then blastula cells（P＜0.05）.With revival blastula cells and gastrulae cells as donors and unfertilized ovums of the Chinese sturgeon as donees, nuclear transplant experiment has been made during which, 469 and 392 ovums were transplanted respectively with a result of 5 and 2 cloned fish, the success rate of nuclear transplant was 1.1% and 0.5%.It proves that genetic resources can be preserved by cryopreserved embryonic cells combined with nuclear transplanting technology. Key words: Acipenser sinensis; embryonic cell; cryopreservation; nuclear transplantation