饲料牛磺酸对尼罗罗非鱼生长、体成分及组织游离氨基酸含量的影响

Effects of taurine supplementation on the growth,body composition and tissue free amino acid concentrations in Nile tilapia(Oreochromis niloticus)

  • 摘要: 本实验旨在探讨饲料牛磺酸水平对尼罗罗非鱼幼鱼生长性能、体成分及游离氨基酸含量的影响。选用体质量为(5.89±0.03)g的尼罗罗非鱼300尾,随机分为5个处理组,每个处理3个重复,每个重复20尾。在基础饲料中分别添加0、0.4%、0.8%、1.2%和1.6%的牛磺酸,配制成5种实验饲料,分别饲喂不同处理组的罗非鱼,饲养周期为56 d。结果表明:0.8%牛磺酸组罗非鱼增重率最高,且显著高于对照组和1.6%组(P<0.05);饲料牛磺酸水平(0.4%~1.6%)提高了罗非鱼摄食率、肝体比和脏体比,但降低了饲料系数(P<0.05)。随着饲料牛磺酸水平的提高,全鱼粗蛋白质和粗脂肪含量随之升高,全鱼水分和粗灰分含量随之降低(P<0.05);以增重率为指标,通过二次曲线回归分析得出尼罗罗非鱼饲料牛磺酸最适需要量为0.75%。罗非鱼血清、肝脏、肌肉和全鱼中牛磺酸含量与饲料牛磺酸含量存在正相关关系,且牛磺酸添加组血清、肝脏、肌肉和全鱼牛磺酸含量显著高于对照组(P<0.05)。罗非鱼肝脏和肌肉中游离氨基酸含量随饲料牛磺酸含量的增加均呈逐渐下降的趋势,且牛磺酸添加组肝脏和肌肉中游离氨基酸含量显著低于对照组(P<0.05)。

     

    Abstract: The nutritional effects of dietary taurine on the growth, body composition and tissue free amino acid concentrations in Nile tilapia were evaluated.Three hundred tilapiasinitial body weight(5.89±0.03)gwere randomly divided into five groups with three replicate tanks at a stock density of twenty fish in each replicate.Five diets were formulated to contain 0, 0.4%, 0.8%, 1.2%, and 1.6% taurine and were allocated to each of the groups of fish twice a day for a feeding period of 56 days.The fish fed the diet at 0.8% taurine got highest weight gain(P<0.05).Dietary taurine levels at 0.4%-1.6% increased feed intake, hepatosomatic index and viscera index, but reduced feed conversion ratio(P<0.05);Both whole-body moisture and ash contents decreased but whole-body crude protein and lipid contents increased with the increase of dietary taurine level(P<0.05);Quadratic curve analysis indicated that the optimal level of dietary taurine for tilapia growth was 0.75% based on weight gain.Taurine concentrations in serum, liver, muscle and whole-body fish were positively linearly correlated with the dietary taurine levels, and the values for these tissues in taurine-treated groups were significantly higher than those of control group(P<0.05).With the increase of dietary taurine, free amino acid concentrations in liver and muscle gradually decreased, and the values in taurine-treated groups were significantly lower than those in control group(P<0.05).

     

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