坛紫菜与Pyropia radi种间杂交重组优良品系的选育与特性分析

Selection and characterization of an improved strain produced by genetic recombinant of interspecific hybridization between Pyropia haitanensis and Pyropia radi

  • 摘要: 为获得具明显杂交重组优势的紫菜优良品系,实验以印度产紫菜Pyropia radi野生型品系(Pr-WT01,特点:藻体厚、生长慢、韧性好、壳孢子放散量多)为父本,坛紫菜诱变品系(Ph-HMC5,特点:藻体薄、生长快、韧性差、壳孢子放散量少)为母本进行种间杂交,从杂合丝状体产生的F1叶状体中选出4个综合性状优良的杂交重组品系,HR-6品系为其中的最优良品系。父本品系叶状体的绝对生长率最大值为0.39 cm/d,母本品系为5.24 cm/d,而HR-6品系高达9.27 cm/d,在日龄30~50 d期间,它的平均绝对生长率比母本品系的最大生长率还大。日龄60 d的叶状体平均体长父本品系为13.18 cm,母本品系为85.67 cm,而HR-6品系已达218.57 cm,分别是父、母本品系的16.58倍和2.55倍。日龄35 d的HR-6品系叶状体的叶绿素a含量高达9.43 mg/g,比父、母本品系分别增加了50%和20%;另外,它的总藻胆蛋白含量高达99.62 mg/g,分别是父、母本品系的2.18倍和1.74倍。日龄35 d的HR-6品系的叶状体平均厚度为26.22 μm,比父本品系降低了37%,比母本品系增加了30%,叶状体的韧性明显增加。HR-6品系的壳孢子放散总量达167.72万个/贝,分别为父、母本品系的1.13和2.65倍。上述研究结果表明,HR-6品系具有生长快、品质好、壳孢子放散量大等优良性状,表现出良好的生产适用性。

     

    Abstract: In this paper, a crossing-experiment between Pyropia radi from India and Pyropia haitanenesis from China was done for breeding of improved strains with obvious hybrid recombinant advantages.The male parent was the wild-type strain(Pr-WT01)of P.radi, which was characterized by thick blade, slow growing, good toughness and high production of conchospores, and the female parent was the mutation strain(Ph-HMC5)of P.haitanensis, which was characterized by thin blade, fast growing, poor toughness and low production of conchospores.At first, four improved strains with obvious hybrid recombinant advantages were obtained from the F1 color-sectored blades, and finally the most improved strain(HR-6)was selected from them.The maximum of absolute growth rate of the male and female parents were 0.39 and 5.24 cm/d, respectively, while that of HR-6 strain was 9.27 cm/d and its absolute growth rate was greater than the maximum growth rate of the female parent during 30-50 days in culture.After 60 days in culture, the mean length of F1 blades of the male and female parents were 13.18 cm and 85.67 cm, respectively, while that of HR-6 strain was 218.57 cm which was 2.55 times that of the female parent and 16.58 times that of the male parent, respectively.The contents of Chl.a in the 35-day-old blades of HR-6 strain was 9.43 mg/g, increasing by 50% and 20% in contrast with that of the male parent and the female parent, respectively.The content of phycobiliprotein of HR-6 strain was 99.62 mg/g, which was 1.74 times that of the female parent and 2.18 times that of the male parent, respectively.The mean thickness of the 35-day-old blades of HR-6 strain was 26.22 μm, decreasing by 37% in contrast with that of the male parent and increasing by 30% in contrast with that of the female parent, and the toughness of the F1 blades of HR-6 strain increased significantly.The total number of conchospores released from HR-6 strain was 167.72×104, which was 2.65 times that of the female parent and 1.13 times that of the male parent, respectively.The above results confirmed that HR-6 was characterized by fast growth, high quality, large amount of conchospores, having excellent production applicability.

     

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