一种杂交鲤四种经济性状的QTL定位分析

Quantitative trait locus analysis of four economic traits in one kind of common carp

  • 摘要: 以柏氏鲤和荷包红鲤抗寒品系杂交F2 1个家系的92尾个体为材料,用300个微卫星(SSR)标记构建了遗传连锁图谱,其长度为2 471.7 cM,标记间平均间隔为10.75 cM,在此基础上对体长(SL)、体厚(BT)、体高(H)和体质量(W)进行QTL定位分析。共检测到17个QTL区间分布于6个连锁群。4个体长的QTL中,位于LG5、LG7和LG19的QTL为显著水平(P<0.05),位于LG32的QTL为极显著水平(P<0.01),可解释表型变异率为5.14%~10.2%;4个体厚的QTL中,位于LG11(两个QTL)和LG32上的QTL为显著水平(P<0.05),位于LG5的QTL达极显著水平(P<0.01),可解释表型变异率为6.42%~8.43%;6个体高的QTL中,LG5,LG10,LG11,LG19,LG32上的QTL为显著水平(P<0.05),LG7上达极显著水平(P<0.01),可解释表型变异率为5.92%~8.95%;3个体质量的QTL中,位于LG5和LG7上的为显著水平(P<0.05),可解释表型变异率为5.55%和8.36%,LG32上达极显著水平(P<0.01),可解释表型变异率为6.44%。本研究采用了两个不同品系的杂交子代为作图群体,丰富了QTL研究群体的多样化,为今后不同鲤品系或品种间的QTL在全基因组水平上的比较和共性QTL的发掘等奠定基础,进而指导鲤分子育种。

     

    Abstract: In this study,the reference panel contained 92 individuals,which were a F2 family obtained by mating of F1 hybrids derived from crossing Barbless carp(Cyprinus pellegrini)with Hebao cold tolerance red carp(Cyprinus carpio wuyuanensis),and the genetic linkage map was constructed with 300 microsatellites,which was used to detect the QTLs for standard length(SL),body thickness(BT),body height(H)and body weight(W)in the panel by GridQTL software.The results showed that 17 related QTLs distributed in the 6 linkage groups were obtained.Four QTLs related to standard length,of which the linkage group of LG5,LG7,LG19 and LG32 were at 5% significant level,but LG32 was at 1% significant level,accounting 5.14%,10.2%,7.02%,9.36% of phenotypic variation,respectively.Four QTLs were identified for body thickness in LG11,LG19,which were at 5% significant level,QTL based on LG5 1% was at significant level,accounting for 6.54%,6.42% and 8.43% of phenotypic variation,respectively.Six QTLs were associated with body height in LG5,LG7,LG10,LG11,LG19 and LG32.LG7 was at 1% significant level,whereas the others were at 5% significant level,accounting 8.95%,6.34%,5.92%,6.29%,6.78%,7.79% of phenotypic variation.Three QTLs were responsible for body weight and LG5,LG7 were at 5% significant level and LG32 was at 1% significant level,which accounted for 6.44%,5.55%,8.36%.Hybrid progenies crossed by two carp strains have been used in this study,which enriched the diversity of QTL population.It is a foundation for further QTL comparative analysis with whole genome level,and common QTL identification in different strains or breeds of carp.It will also be of great assistance in marker assisted selection(MAS)in common carp.

     

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