脊尾白虾3个野生群体遗传多样性的微卫星分析

Microsatellite analysis of genetic diversity in 3 wild populations of Exopalaemon carinicauda

摘要: 本文利用12对微卫星标记分析了莱州湾(LZ)、海州湾(HZ)、象山(XS)脊尾白虾野生群体的遗传多样性。12个位点在3个群体中均表现出高度的多态性。12个微卫星位点共得到115个等位基因,各个位点的等位基因数介于6-14之间,平均每个位点上的等位基因数为9.5833个；各个位点的平均期望杂合度(He)、平均观测杂合度(Ho)、平均多态信息含量(PIC)分别为0.8278、0.5175、0.7051,表明3个脊尾白虾野生群体均有良好的多态性。经F-统计分析,各个群体间的遗传分化指数均值为0.0930 (0.05

Abstract: Genetic diversity of 3 wild stocks,collected from Laizhuo Bay(LZ),Haizhuo Bay(HZ),Xiangshang(XS), were investigated using 12 microsatellite loci. A total of 115 alleles were detected in 12 microsatellite loci and the number of alleles ranged from 6 to 14(mean 9.5833) . The expected and observed heterozygosities in 12 loci ranging from 0.7653 to 0.8660 (mean 0.8278) and from 0.2167 to 0.7176 (mean 0.5175), respectively. The average polymorphic information content of 12 loci was 0.7051. Mean coefficient of gene differentiation for loci( Fst ) was 0.0930(0.05