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条石鲷线粒体COⅠ和Cytb序列的遗传变异分析

Genetic variation of COⅠ and Cytb sequences in Oplegnathus fasciatus

    摘要
  • 摘要: 通过PCR扩增与测序分别获得了长度为642 bp和1 138 bp的条石鲷线粒体COⅠ和Cytb基因片段。分析表明,COⅠ序列共定义了11个单倍型,存在21个多态性位点,发生转换4次,颠换5次。A、T、G、C碱基的平均含量分别为24.5%、30.6%、18.8%和26.1%。Cytb序列共定义了11个单倍型,存在26个多态性位点,发生转换4次,颠换3次。A、T、G、C碱基的平均含量分别为24.9%、28.3%、14.8%和32.0%。基于COⅠ和Cytb两基因序列的单倍型多样性(Hd)、核苷酸多样性(Pi)和平均碱基差异(K)分别为 0.795、0.008 83、5.667和 0.770、0.003 54、4.025。结果表明,条石鲷群体的COⅠ和Cytb基因片段显示出较高的遗传多样性水平。本研究结果可为条石鲷资源保护及系统进化研究提供基础资料。

     

    Abstract: Using PCR amplification and sequencing,642 bp of cytochrome c oxidase subunitⅠ(COⅠ)partial sequences and 1 138 bp of Cytb partial sequences were obtained from Oplegnathus fasciatus.In COⅠ sequences,a total of 21 polymorphic sites defined 11 distinct haplotypes.And 4 transition loci,5 transversion loci and no insertions and deletions were found in them.The average content of A,T,G and C was 24.5%,30.6%,18.8%,and 26.1%,respectively.In Cytb sequences,26 polymorphic sites and 11 haplotypes were defined,and 4 transition loci and 3 transversion loci were found.The average content of A,T,G and C was 24.9%,28.3%,14.8% and 32.0%,respectively.The mean haplotype diversity(Hd),nucleotide diversity(Pi),and the average number of nucleotide difference(K)in COⅠ and Cytb sequences were 0.795,0.008 83,5.667 and 0.770,0.003 54,4.025,respectively.The results suggested that both COⅠ and Cytb gene showed high level of genetic diversity in O.fasciatus.These results provide basic information for the genetic resource protection and phylogenetic study of this species.

     

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