Abstract:
In this study, full-length cDNA of
Os-IP3R and
Os-RyR in
Onchidium struma was cloned by transcriptome data mining and rapid amplification cDNA ends (RACE) methods, and we analyzed the relationship between the percentages of
Os-IP3R/
Os-RyR expression and the evolution of 3 species from family Onchidiidae. The bioinformatic analysis and expression pattern of
Os-IP3R and
Os-RyR in different tissues from
Onchidium struma,
Platevindex mortoni and
Peronia verruculata detected by Real-time quantitative PCR were performed. The results show that length cDNA of
Os-IP3R consists of 4 574 base pairs (bp), and 2 808 bp open reading frame (ORF) which encodes 935 amino acids. The predicted moleculer of the deduced amino acid of
IP3R has six transmembrane domains. The length cDNA of
Os-RyR consists of 1 253 base pairs (bp), and 1 131 bp open reading frame (ORF) which encodes 376 amino acids. The predicted molecular of the deduced amino acid of
RyR has three transmembrane domains. The
IP3R and
RyR amino acid sequences in
O. struma were aligned and found to be highly conserved in the G*R*GGG*GD at region of calcium channel.
Os-IP3R and
Os-RyR were detected by qRT-PCR in different tissues of three kinds of Onchidiidae, and the percentages of
Os-IP3R/
Os-RyR expression from three species were consistent with their living environment (from high tide zone to shallow sea), followed by
O. struma,
P. mortoni and
P. verruculata. The species of higher terrestrial ability with higher percentages of
Os-IP3R/
Os-RyR gene expression, illustrated percentage of gene expression associated with evolutionary degree. According to the comparison of percentage in Onchidiidae, we hope to provide a valuable clue for amphibian investigators, and to give a molecular biology clue for studying sea to land transition of marine invertebrate.
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