3个暗紫红毛菜种群的形态、繁殖方式及染色体研究

Study of morphology,reproduction and chromosomes of three Bangia atropurpurea(Bangiales,Rhodophyta)populations

  • 摘要: 为了解我国暗紫红毛菜(淡水红毛菜)的生物学特征,对采自山西娘子关、兰州五泉山、兰州兴隆山的暗紫红毛菜进行了形态、繁殖方式、染色体及色素含量等观察研究。观察采集现场暗紫红毛菜生长环境特征,显微镜观察记录各藻体形态特征,通过原叶体的培养观察各种群的繁殖方式,并用醋酸苏木精染色法观察核分裂过程。结果显示,3个采集地暗紫红毛菜群落均呈点状和不连续性分布,并仅限于生长在瀑布冲击和流水飞溅下的岩石或山溪流水有落差之处有分布,常与疏枝刚毛藻混生在一起。红毛菜均呈不分枝细丝状,长度在1.1~3.5 cm。样品藻胆蛋白含量在7.26~9.46 mg/g之间,娘子关暗紫红毛菜藻胆蛋白含量最高,其次为五泉山红毛菜和兴隆山红毛菜。3个暗紫红毛菜种群繁殖方式均以放散单孢子进行无性生殖维持种群的繁衍。原叶体细胞核分裂过程均为有丝分裂,从前期向中期转变过程中,均呈现姐妹染色单体交叉呈"8"型或"X"型。染色体数均为n=4,染色体长度在0.42~1.67 μm之间,其中3条长度相近,1条略短。五泉山和兴隆山暗紫红毛菜形态、繁殖方式及染色体为首次报道。本实验为我国暗紫红毛菜研究积累了基础资料。

     

    Abstract: Bangia atropurpurea has been inadequately studied in China.To understand the basic biological characteristics of B.atropurpurea in China, the morphology, reproduction, chromosome characteristics and pigment contents of B.atropurpurea were studied in this research.Three B.atropurpurea samples were collected from Niangziguan(Shanxi Province), Wuquanshan(Gansu Province)and Xinglongshan(Gansu Province).The growth environment conditions of the Bangia samples were studied in the fields.The morphological and reproduction characteristics of these three samples were examined under the light microscope.The nuclear division processes of samples were observed under a photomicroscope with an aceto-iron-haematoxylin-chloral hydrate stain.The freshwater Bangia populations in these three different locations were found adhered to rocks, waterfall and fast flowing streams, and the Bangia populations were often found to coexist with Cladophora insignis.These Bangia samples were unbranched filaments and the length ranged from 1.1 cm to 3.5 cm.Phycobiliprotein contents of three samples were ranged from 7.26 to 9.46 mg/g, in which the sample from Niangziguan contained the highest concentration, followed by the sample from Wuquanshan.The Xinlongshan sample contained the lowest phycobiliprotein contents concentration in comparison with other samples.These Bangia samples reproduced asexually and the released archeospores germinated into gametophytes continously.The nuclear division of three Bangia samples were mitosis and the sister chromosomes always assembled as a "8" or a "X" shape in the early metaphase.All freshwater samples had the consistent somatic chromosome numbers of n=4.The length of chromosomes were ranged from 0.42 to 1.67 μm and one of the chromosomes was usually found to be shorter than the other three.This is the first time that the biology characteristics of Bangia samples from Wuquanshan and Xinglongshan have been systematically studied in China.That provides useful information for the B.atropurpurea research.

     

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