Abstract: The purpose of this study was to investigate the effects of prepared hydrolysis peptides-Fe²⁺ complexes(TPH-Fe²⁺)on the depuration of cadmium(Cd²⁺)from red swamp crayfish(P.clarkii)tissues.The results indicated that the concentration of Cd²⁺ in crayfish tissues from high to low was visceral mass>intestine>gill>abdomen muscle>cheliped muscle respectively,all of which were not significantly decreased during the 12 days of depuration.However,significant differences were observed in groups feeding by 400,800,and 1 200 mg/kg TPH-Fe²⁺added into basal feed after 12 day's feeding.The Cd²⁺ concentrations in intestine,visceral mass,gill,and abdomen muscle were significantly decreased by 40.60%,36.13%,33.36%,and 10.15%,respectively.Nevertheless,the variables of Cd²⁺ concentrations in heliped muscle treated with 400-1 200 mg/L TPH-Fe²⁺ showed no significant differences from controls at any point during the experimental period.For these reasons,TPH-Fe²⁺ can be recommended as depuration agent in crayfish feed to decrease the Cd²⁺ concentrations in tissues.